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Project:Bioactive peptides
Description:Bioactive peptides from urban pruning

Selected functional unit
QuantityUnitFunctional unit description
1kg of bioactive peptidesProduction of one kilogram of purified bioactive peptides from pruning.

Input materials
QuantityUnitDescription
65kgUrban pruning biomass residue
480kgProcess water for extraction and washing
0.75kgProtease enzymes for enzymatic hydrolysis
3.2kgSodium hydroxide for pH control
2.8kgHydrochloric acid for pH neutralization
10.5kgAmmonium sulfate for protein precipitation
12kgEthanol for peptide purification and rinsing
Output materials
QuantityUnitDescription
64kgSpent urban pruning biomass residue after peptide extraction
480kgWastewater from extraction and washing processes
12kgSpent ethanol solvent from purification and rinsing steps
10.5kgResidual ammonium sulfate from the protein precipitation stage
6kgDissolved salts formed from pH neutralization
0.75kgDegraded protease enzymes remaining in the waste stream
Energy inputs
QuantityUnitDescription
9.75kWhMechanical milling and grinding of urban pruning residues
18.2kWhHeating and agitation for extraction and enzymatic hydrolysis
5.4kWhCentrifugation for separation of protein-rich supernatant
7.6kWhUltrafiltration and ethanol purification of bioactive peptides
42.5kWhFreeze-drying for final stabilization of purified peptide powder
Energy outputs
QuantityUnitDescription
0kWhNo energy produced
CO2eq emitted
QuantityUnitDescription
4.39Kg CO2eqMechanical milling and grinding of urban pruning residues
8.19Kg CO2eqHeating and agitation for extraction and enzymatic hydrolysis
2.43Kg CO2eqCentrifugation for separation of protein-rich supernatant
3.42Kg CO2eqUltrafiltration and ethanol purification of bioactive peptides
19.13Kg CO2eqFreeze-drying for final stabilization of purified peptide powder
0Kg CO2eqNo CO2eq emitted directly
Captured CO2eq
QuantityUnitDescription
0Kg CO2eqNo energy produced
119.18Kg CO2eqBiogenic CO2 sequestration in urban pruning biomass residue

Production of one kilogram of purified bioactive peptides from pruning.